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CC-4741 rbcS1-ABGP mt-

Name: CC-4741 rbcS1-ABGP mt-
SKU: CC-4741
Availability: InStock

$30.00

From Robert J. Spreitzer, University of Nebraska, July 2014

Phenotype: requires acetate at 35 °C, temperature-conditional

Using standard methods of genetic engineering and nuclear-gene transformation of rbcS∆-T60-3 mt- (CC-4690) (Genkov et al. 2010), the betaA-betaB loop of the Chlamydomonas Rubisco small subunit was replaced with the loop from Galdieria partita Rubisco, which causes a decrease in Rubisco holoenzyme stability (Genkov and Spreitzer, unpublished). The mutant can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.

Genkov T, Meyer M, Griffiths H, Spreitzer RJ (2010) Functional hybrid Rubisco enzymes with plant small subunits and algal large subunits: Engineered rbcS cDNA for expression in Chlamydomonas. J Biol Chem 285:19833-19841

Locus:
RBCS1
Chromosome:
2

CC-4741 rbcS1-ABGP mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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