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CC-4746 rbcS1-L18A mt-

Name: CC-4746 rbcS1-L18A mt-
SKU: CC-4746
Availability: InStock

$30.00

From Robert J. Spreitzer, University of Nebraska, July 2014

Phenotype: requires acetate at 35 °C, temperature-conditional

Directed mutagenesis and nuclear-gene transformation of rbcS∆-T60-3 mt- (CC-4415) were used to create an L18A substitution (CTG-GCC) in the Rubisco small subunit, which causes a decrease in Rubisco holoenzyme stability (Genkov and Spreitzer 2009). This mutant can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.

Genkov T, Spreitzer RJ (2009) Highly conserved small subunit residues influence Rubisco large subunit catalysis. J Biol Chem 284:30105-30112

Locus:
RBCS1
Chromosome:
2

CC-4746 rbcS1-L18A mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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