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CC-4750 rbcS1-Y32E mt-

Name: CC-4750 rbcS1-Y32E mt-
SKU: CC-4750
Availability: InStock

$30.00

From Robert J. Spreitzer, University of Nebraska, July 2014

Phenotype: requires acetate at 35 °C, temperature-conditional

Using established methods of directed mutagenesis and nuclear-gene transformation of rbcS∆-T60-3 mt- (CC-4415) (Genkov and Spreitzer 2009), Todor Genkov in Spreitzer’s group created a Y32E substitution in the Rubisco small subunit, which causes a decrease in Rubisco CO2/O2 specificity and holoenzyme stability (Genkov and Spreitzer, unpublished). This mutant can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.

Genkov T, Spreitzer RJ (2009) Highly conserved small subunit residues influence Rubisco large subunit catalysis. J Biol Chem 284:30105-30112

Locus:
RBCS1
Chromosome:
2

CC-4750 rbcS1-Y32E mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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