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CC-4754 rbcS1-E43A pf2 mt+

Name: CC-4754 rbcS1-E43A pf2 mt+
SKU: CC-4754
Availability: InStock

$30.00

Phenotype: requires acetate at 35 °C, temperature-conditional

Using a plasmid that would create an E43A substitution (GAG-GCC) in the Rubisco small subunit, which causes a decrease in Rubisco CO2/O2 specificity and holoenzyme stability (Genkov and Spreitzer 2009), Todor Genkov in Spreitzer’s group transformed rbcS∆-CAL005.01.13 pf2 mt+ (CC-4692). In contrast to the E43A mutant described previously (Genkov and Spreitzer 2009), this strain has a cell wall and can be used in genetic crosses. This mutant can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.

Genkov T, Spreitzer RJ (2009) Highly conserved small subunit residues influence Rubisco large subunit catalysis. J Biol Chem 284:30105-30112

Locus:
RBCS1, PF2
Chromosome:
2,11

CC-4754 rbcS1-E43A pf2 mt+

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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