* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4758 rbcS1-F81A mt-
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Directed mutagenesis and nuclear-gene transformation of rbcS∆-T60-3 mt- (CC-4415) were used to create an F81A substitution (TTC-GCC) in the Rubisco small subunit, which causes a decrease in Rubisco carboxylation catalytic efficiency and holoenzyme stability (Genkov and Spreitzer 2009). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Genkov T, Spreitzer RJ (2009) Highly conserved small subunit residues influence Rubisco large subunit catalysis. J Biol Chem 284:30105-30112