* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4765 rbcS1-SSHA mt-
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Phenotype: altered CO2 assimilation
Genetic engineering and nuclear-gene transformation of rbcS∆-T60-3 mt- (CC-4415) were used to create a hybrid Rubisco enzyme comprised of Chlamydomonas large subunits and sunflower small subunits (Genkov et al. 2010). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Genkov T, Meyer M, Griffiths H, Spreitzer RJ (2010) Functional hybrid Rubisco enzymes with plant small subunits and algal large subunits: Engineered rbcS cDNA for expression in Chlamydomonas. J Biol Chem 285:19833-19841