CC-4767 rbcS1-SSNT mt-
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Phenotype: altered CO2 assimilation
Genetic engineering and nuclear-gene transformation of rbcS∆-T60-3 mt- (CC-4415) (Genkov et al. 2010) were used to create a hybrid Rubisco enzyme comprised of Chlamydomonas large subunits and tobacco small subunits for studies of Rubisco activase (Wachter et al. 2013). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Genkov T, Meyer M, Griffiths H, Spreitzer RJ (2010) Functional hybrid Rubisco enzymes with plant small subunits and algal large subunits: Engineered rbcS cDNA for expression in Chlamydomonas. J Biol Chem 285:19833-19841
Wachter RM, Salvucci ME, Carmo-Silva AE, Barta C, Genkov T, Spreitzer RJ (2013) Activation of interspecies-hybrid Rubisco enzymes to assess different models for the Rubisco-Rubisco activase interaction. Photosynth Res 117:557-566