CC-4801 rbcL-V331A/T342I mt+ (R40-9D revertant of 45-3B)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
R40-9D was recovered as a photosynthesis-competent revertant of rbcL-V331A mt+ (45-3B) (Chen and Spreitzer 1989). It results from an intragenic-suppressor mutation that causes a T342I substitution (ACT-ATT) in the Rubisco large subunit, and the double-mutant enzyme has decreases in Rubisco CO2/O2 specificity and holoenzyme stability (Chen and Spreitzer 1989; Chen et al. 1991). The V331A/T342I mutant-enzyme x-ray crystal structure has been solved (Karkehabadi et al. 2007). The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Chen Z, Spreitzer RJ (1989) Chloroplast intragenic suppression enhances the low CO2/O2 specificity of mutant ribulosebisphosphate carboxylase/oxygenase. J Biol Chem 264:3051-3053
Chen Z, Yu W, Lee JH, Diao R, Spreitzer RJ (1991) Complementing amino-acid substitutions within loop 6 of the alpha/beta-barrel active site influence the CO2/O2 specificity of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase. Biochemistry 30:8846-8850
Karkehabadi S, Satagopan S, Taylor TC, Spreitzer RJ, Andersson I (2007) Structural analysis of altered large-subunit loop-6/carboxy-terminus interactions that influence catalytic efficiency and CO2/O2 specificity of ribulose-1,5-bisphosphate carboxylase/oxygenase. Biochemistry 46:11080-11089