CC-4809 rbcL-V331A/I393M mt+ (R258-3B revertant of 45-3B)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
R258-3B was recovered as a photosynthesis-competent revertant of rbcL-V331A mt+ (45-3B) (Chen and Spreitzer 1989; Chen et al. 1991). It results from intragenic suppression that causes an I393M substitution in the Rubisco large subunit (Satagopan and Spreitzer, unpublished). The double-mutant strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Chen Z, Spreitzer RJ (1989) Chloroplast intragenic suppression enhances the low CO2/O2 specificity of mutant ribulosebisphosphate carboxylase/oxygenase. J Biol Chem 264:3051-3053
Chen Z, Yu W, Lee JH, Diao R, Spreitzer RJ (1991) Complementing amino-acid substitutions within loop 6 of the alpha/beta-barrel active site influence the CO2/O2 specificity of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase. Biochemistry 30:8846-8850