CC-4815 rbcL-G237S pf2 mt- (67-2AA) (pf2 progeny used for genetic analysis)
$30.00
From Robert J. Spreitzer, University of Nebraska, 2014
Phenotype: requires acetate, sensitive to light
This chloroplast mutant was isolated by screening a large collection of acetate-requiring mutants (Spreitzer et al. 1988) for lack of complementation (Spreitzer and Ogren 1983) with a known rbcL mutant. The mutant was crossed with pf2 mt- to confirm uniparental inheritance, and an mt- progeny clone was analyzed (Thow and Spreitzer 1991; Spreitzer 1993). The mutant results from a GGT to AGT rbcL mutation that causes Gly-237 to be substituted by Ser in the Rubisco large subunit. The mutant strain has a decreased level of Rubisco holoenzyme and lacks carboxylase activity. Attempts to select photosynthesis-competent revertants yielded only true revertants (Spreitzer 1998). This strain has been maintained with acetate medium in darkness since its recovery.
Spreitzer RJ (1998) Genetic engineering of Rubisco. In JD Rochaix, M Goldschmidt-Clermont, S Merchant, eds, The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas, Kluwer Academic Publishers, Dordrecht, pp 515-527
Spreitzer RJ, Ogren WL (1983) Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 80:6293-6297
Spreitzer RJ, Al-Abed SR, Huether MJ (1988) Temperature-sensitive, photosynthesis-deficient mutants of Chlamydomonas reinhardtii. Plant Physiol 86:773-777
Thow G, Spreitzer RJ (1991) Decreased accumulation of Rubisco due to a chloroplast rbcL mutation of Chlamydomonas. Plant Physiol 96:49
Spreitzer RJ (1993) Genetic dissection of Rubisco structure and function. Annu Rev Plant Physiol Plant Mol Biol 44:411-434