CC-4816 rbcL-Q45Ocher mt+ (68-7AN) (original isolate)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Phenotype: requires acetate, sensitive to light
The 68-7AN chloroplast mutant was recovered by screening a collection of acetate-requiring mutants (Spreitzer et al. 1988) for lack of complementation with a known Rubisco rbcL mutant (Spreitzer and Ogren 1983). It results from an rbcL nonsense mutation that changes the codon for Gln-45 (CAA) to ocher (TAA) (Spreitzer 1993). It is not known whether this mutant may be suppressed by heteroplasmic informational (tRNA) suppression (see rbcL-W66Amber) (Yu and Spreitzer 1992). This is the original mutant strain. It has been maintained with acetate medium in darkness since its isolation.
Spreitzer RJ (1993) Genetic dissection of Rubisco structure and function. Annu Rev Plant Physiol Plant Mol Biol 44:411-434
Spreitzer RJ, Al-Abed SR, Huether MJ (1988) Temperature-sensitive, photosynthesis-deficient mutants of Chlamydomonas reinhardtii. Plant Physiol 86:773-777
Spreitzer RJ, Ogren WL (1983) Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 80:6293-6297
Yu W, Spreitzer RJ (1992) Chloroplast heteroplasmicity is stabilized by an amber-suppressor tryptophan tRNA(CUA). Proc Natl Acad Sci USA 89:3904-3907