CC-4818 rbcL-Q45Ocher mt- (68-7AN) (non-pf2 progeny used for genetic analysis)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Phenotype: requires acetate, sensitive to light
The 68-7AN chloroplast mutant was recovered by screening a collection of acetate-requiring mutants (Spreitzer et al. 1988) for lack of complementation with a known Rubisco rbcL mutant (Spreitzer and Ogren 1983). It results from an rbcL nonsense mutation that changes the codon for Gln-45 (CAA) to ocher (TAA) (Spreitzer 1993). It is not known whether this mutant may be suppressed by heteroplasmic informational (tRNA) suppression (see rbcL-W66Amber) (Yu and Spreitzer 1992). This strain was recovered from a cross between the original 68-7AN mt+ and pf2 mt-, and has been maintained with acetate medium in darkness since its isolation.
Spreitzer RJ (1993) Genetic dissection of Rubisco structure and function. Annu Rev Plant Physiol Plant Mol Biol 44:411-434
Spreitzer RJ, Al-Abed SR, Huether MJ (1988) Temperature-sensitive, photosynthesis-deficient mutants of Chlamydomonas reinhardtii. Plant Physiol 86:773-777
Spreitzer RJ, Ogren WL (1983) Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 80:6293-6297
Yu W, Spreitzer RJ (1992) Chloroplast heteroplasmicity is stabilized by an amber-suppressor tryptophan tRNA(CUA). Proc Natl Acad Sci USA 89:3904-3907