CC-4824 rbcL-N123G mt- (non-pf2 progeny used for genetic analysis)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Phenotype: requires acetate, sensitive to light
Directed mutagenesis and chloroplast co-transformation of wild-type 2137 mt+ (CC-3269) were used to create an N123G substitution (AAC-GGC) in the Rubisco large subunit, which causes a decrease in Rubisco CO2/O2 specificity but not holoenzyme stability (Zhu and Spreitzer 1994). It was created due to a previous study of Rhodospirillum rubrum Rubisco (Chene et al. 1992). This strain was recovered from a cross between the original N123G mt+ and pf2 mt-, and has been maintained with acetate medium in darkness since its isolation.
Chene P, Day AG, Fersht AR (1992) Mutation of asparagine 111 of Rubisco from Rhodospirillum rubrum alters the carboxylase/oxygenase specificity. J Mol Biol 225:891-896
Zhu G, Spreitzer RJ (1994) Directed mutagenesis of chloroplast ribulose-bisphosphate carboxylase/oxygenase: Substitutions at large subunit asparagine 123 and serine 379 decrease CO2/O2 specificity. J Biol Chem 269:3952-3956