CC-4827 rbcL-S379A pf2 mt- (pf2 progeny used for genetic analysis)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Phenotype: requires acetate, sensitive to light
Directed mutagenesis and chloroplast co-transformation of wild-type 2137 mt+ (CC-3269) were used to create an S379A substitution (TCA-GCA) in the Rubisco large subunit, which causes a decrease in Rubisco CO2/O2 specificity but not holoenzyme stability (Zhu and Spreitzer 1994). It was created due to previous studies of Synechococcus and Rhodospirillum rubrum Rubisco (Harpel and Hartman 1992; Lee and McFadden 1992). This strain was recovered from a cross between the original S379A mt+ and pf2 mt-, and has been maintained with acetate medium in darkness since its isolation.
Harpel MR, Hartman FC (1992) Enhanced CO2/O2 specificity of a site-directed mutant of ribulosebisphosphate carboxylase/oxygenase. J Biol Chem 267:6475-6478
Lee GJ, McFadden BA (1992) Serine-376 contributes to the binding of substrate by ribulose-bisphosphate carboxylase/oxygenase from Anacystis nidulans. Biochemistry 31:2304-2308
Zhu G, Spreitzer RJ (1994) Directed mutagenesis of chloroplast ribulose-bisphosphate carboxylase/oxygenase: Substitutions at large subunit asparagine 123 and serine 379 decrease CO2/O2 specificity. J Biol Chem 269:3952-3956