CC-4846 rbcL-C256F/K258R/I265V mt+ (Triple)
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
A plasmid containing the rbcL-C256F/K258R/I265V gene (Du et al. 2003) was transformed into rbcL∆-MX3312 mt+ (CC-4696) (Spreitzer et al. 2005). This strain was created for isogenic comparison with rbcL-V221C/V235I/C256F/K258R/I265V mt+ (Penta) (Spreitzer et al. 2005). The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Du YC, Peddi SR, Spreitzer RJ (2003) Assessment of structural and functional divergence far from the large subunit active site of ribulose-1,5-bisphosphate carboxylase/oxygenase. J Biol Chem 278:49401-49405
Spreitzer RJ, Peddi SR, Satagopan S (2005) Phylogenetic engineering at an interface between large and small subunits imparts land-plant kinetic properties to algal Rubisco. Proc Natl Acad Sci USA 102:17225-17230