CC-4859 rbcL-L290F/rbcS1-WT mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Phenotype: requires acetate at 35 °C, temperature-conditional
This strain was created by Todor Genkov in Spreitzer’s group by transforming rbcL-L290F/rbcS∆-CAL005.01.13 mt+ with the pSS1 plasmid that contains the Rubisco rbcS1 small-subunit gene (Khrebtukova and Spreitzer 1996). Like the original rbcL-L290F mutant (Chen et al. 1988), it can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. The mutant was created for comparison with rbcL-L290F/rbcS1-L66G mt+. It has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Chen Z, Chastain CJ, Al-Abed SR, Chollet R, Spreitzer RJ (1988) Reduced CO2/O2 specificity of ribulose-1,5-bisphosphate carboxylase/oxygenase in a temperature-sensitive chloroplast mutant of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 85:4696-4699
Khrebtukova I, Spreitzer RJ (1996) Elimination of the Chlamydomonas gene family that encodes the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 93:13689-13693