CC-4865 rbcS1-L66G mt-
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Using standard methods of directed mutagenesis and nuclear-gene transformation of rbcS∆-T60-3 mt- (CC-4690) (Genkov and Spreitzer 2009), Todor Genkov in Spreitzer’s group created an L66G substitution (CTG-GGC) in the Rubisco small subunit. This mutant was created to see whether an L66G substitution in the nuclear-encoded small subunit could suppress the biochemical defects that arise from an L290F substitution in the chloroplast-encoded large subunit (Chen et al. 1988). The L66G substitution increases the thermal stability of wild-type Rubisco (Genkov and Spreitzer, unpublished). This strain has been maintained with acetate medium in darkness since its creation.
Chen Z, Chastain CJ, Al-Abed SR, Chollet R, Spreitzer RJ (1988) Reduced CO2/O2 specificity of ribulose-1,5-bisphosphate carboxylase/oxygenase in a temperature-sensitive chloroplast mutant of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 85:4696-4699
Genkov T, Spreitzer RJ (2009) Highly conserved small subunit residues influence Rubisco large subunit catalysis. J Biol Chem 284:30105-30112