CC-4874 rbcL-Q401T mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Using standard methods of directed mutagenesis and chloroplast transformation of rbcL∆-MX3312 mt+ (CC-4696) (Satagopan and Spreitzer 2004), a Q401T substitution (CAG-ACT) was created in the Rubisco large subunit. This mutant was created to investigate phylogenetic differences in the Rubisco active site (Spreitzer et al. 1988; Chene et al. 1997). See also rbcL-T173I (CC-4819). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
William Zerges reported CC-4874 (Q401T) has no Rbcl gene. The pcr band is the same size as the deletion mutant from three trials/independent preps. (personal communication, 12/8/20)
Chene P, Day AG, Fersht AR (1997) Role of isoleucine-164 at the active site of Rubisco from Rhodospirillum rubrum. Biochem Biophys Res Com 232:482-486
Satagopan S, Spreitzer RJ (2004) Substitutions at the Asp-473 latch residue of Chlamydomonas ribulosebisphosphate carboxylase/oxygenase cause decreases in carboxylation efficiency and CO2/O2 specificity. J Biol Chem 279:14240-14244
Spreitzer RJ, Brown T, Chen Z, Zhang D, Al-Abed SR (1988) Missense mutation in the Chlamydomonas chloroplast gene that encodes the Rubisco large subunit. Plant Physiol 86:987-989