CC-4876 rbcL-A222S mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Using standard methods of directed mutagenesis and chloroplast transformation of rbcL∆-MX3312 mt+ (CC-4696) (Satagopan and Spreitzer 2004), an A222S substitution was created in the Rubisco large subunit. This mutant was created to investigate phylogenetic differences in the Rubisco large subunit that may be responsible for pyrenoid formation in algae (Nozaki et al. 2002). See also rbcL-R32K/A222S/C247S. The A222S substitution does not affect Rubisco catalysis or pyrenoid formation (Lim and Meyer, unpublished). An A222T substitution suppresses the low CO2/O2 specificity and thermal instability of rbcL-L290F Rubisco (Du and Spreitzer 2000). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Du YC, Spreitzer RJ (2000) Suppressor mutations in the chloroplast-encoded large subunit improve the thermal stability of wild-type ribulose-1,5-bisphosphate carboxylase/oxygenase. J Biol Chem 275:19844-19847
Nozaki H, Onishi K, Morita E (2002) Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (volvocales, chlorophyceae). J Mol Evol 55:414-430
Satagopan S, Spreitzer RJ (2004) Substitutions at the Asp-473 latch residue of Chlamydomonas ribulosebisphosphate carboxylase/oxygenase cause decreases in carboxylation efficiency and CO2/O2 specificity. J Biol Chem 279:14240-14244