CC-4888 pf2 mt- [Spreitzer]
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
This centromere-marker strain was obtained from crosses between wild-type 2137 mt+ (original) and pf2 mt-, which was received by Laurens Mets from Paul Levine in the 1970s. This is the strain most often used for genetic analysis in Spreitzer’s group (Spreitzer and Mets 1981; Spreitzer and Ogren 1983; Spreitzer et al. 1988; Gotor et al. 1994; Hong and Spreitzer 1994). The pf2 phenotype is best observed by placing a loop of water on a colony, and watching for swimming with a dissecting microscope. This strain has been maintained with acetate medium in darkness since its recovery.
Gotor C, Hong S, Spreitzer RJ (1994) Temperature-conditional nuclear mutation of Chlamydomonas reinhardtii decreases the CO2/O2 specificity of chloroplast ribulose-bisphosphate carboxylase/oxygenase. Planta 193:313-319
Hong S, Spreitzer RJ (1994) Nuclear mutation inhibits expression of the chloroplast gene that encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Plant Physiol 106:673-678
Spreitzer RJ, Al-Abed SR, Huether MJ (1988) Temperature-sensitive, photosynthesis-deficient mutants of Chlamydomonas reinhardtii. Plant Physiol 86:773-777
Spreitzer RJ, Mets L (1981) Photosynthesis-deficient mutants of Chlamydomonas reinhardtii with associated light-sensitive phenotypes. Plant Physiol 67:565-569
Spreitzer RJ, Ogren WL (1983) Nuclear suppressors of the photosensitivity associated with defective photosynthesis in Chlamydomonas reinhardtii. Plant Physiol 71:35-39