CC-4890 11-6F mt+ (original isolate)
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Phenotype: requires acetate, leaky
Following 5-fluorodeoxyuridine treatment and ethyl-methanesulfonate mutagenesis of wild-type 2137 mt+ cells, colonies were screened for light-sensitive, acetate-requiring phenotypes (Spreitzer and Mets 1981). Mutant 11-6F grows slowly on minimal medium in the light and displayed a mendelian pattern of inheritance. It has PSI-deficient fluorescence-induction kinetics, but only a 50% decrease in CO2 fixation (Spreitzer and Mets 1981). This is the original isolate (see also CC-1229 11-6F mt-). It has been maintained with acetate medium in darkness since 1980 to prevent selection for revertants or suppressors.
Spreitzer RJ, Mets L (1981) Photosynthesis-deficient mutants of Chlamydomonas with associated light-sensitive phenotypes. Plant Physiol 67:565-569