CC-4895 10-2E mt- (psp-u-1-10-2E) [formerly CC-2045]
$30.00
From Robert J. Spreitzer, University of Nebraska, August 2014
Phenotype: requires acetate, sensitive to light
Following 5-fluorodeoxyuridine treatment and ethyl-methanesulfonate mutagenesis of wild-type 2137 mt+ cells, colonies were screened for light-sensitive, acetate-requiring phenotypes (Spreitzer and Mets 1981). Mutant 10-2E displayed uniparental inheritance, and has normal fluorescence-induction kinetics (Spreitzer and Mets 1981). Recombination tests showed that 10-2E is at the same locus (psp-u-1) as 8-31, 8-35, 10-7C, and 12-10B (Spreitzer and Ogren 1983a) indicating that it may lack photosynthetic phosphorylation due to an atp-gene mutation (Selman-Reimer et al. 1981). A different photosynthetic phosphorylation locus (psp-u-2) was defined by mutant 12-5A (CC-2260) (Spreitzer and Ogren 1983a). This strain was recovered from a cross between the original 10-2E mt+ and pf2 mt- (from Levine), and has been maintained with acetate medium in darkness since 1980 to prevent selection for revertants and non-light-sensitive suppressors (Spreitzer and Ogren 1983b).
Chromosome: chloroplast
Selman-Reimer S, Merchant S, Selman BR (1981) Isolation and characterization of the chloroplast coupling factor 1 from wild-type Chlamydomonas reinhardi. In BR Selman, S Selman-Reimer, eds, Energy Coupling in Photosynthesis, pp 341-352
Spreitzer RJ, Mets L (1981) Photosynthesis-deficient mutants of Chlamydomonas with associated light-sensitive phenotypes. Plant Physiol 67:565-569
Spreitzer RJ, Ogren WL (1983a) Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 80:6293-6297
Spreitzer RJ, Ogren WL (1983b) Nuclear suppressors of the photosensitivity associated with defective photosynthesis in Chlamydomonas reinhardtii. Plant Physiol 71:35-39