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CC-705 ac46 mt+

Name: CC-705 ac46 mt+
SKU: CC-705
Availability: InStock

$30.00

Chlamydomonas Genetics Center, Duke University, 1978

Phenotype: requires acetate (suppressed)

Strains carrying the ac46 mutation have a strong tendency to acquire second-site suppressors, but we have always been able to recover the original mutation by outcrossing to wild type. The y1 mutant is a better genetic marker for this linkage group.

CC-705 is from CC-125 x CC-563, a suppressed ac46 strain no longer in the collection. It was scored as cleanly acetate-requiring in 1978, In a cross in 1982 of CC-705 x CC-124, complete tetrads were obtained with clear 2:2 segregation. CC-705 was subsequently outcrossed to produce CC-1361.

The ac46 mutant appears to map within 1-2 map units of its own centromere but in early crosses did not show linkage to any of 36 markers tested on other linkage groups. Crosses by Dutcher et al. and by the Chlamydomonas Genetics Center subsequently showed that AC46 and Y1 constitute a single linkage group.

Levine and Goodenough listed the ac46 mutant as non-photosynthetic; the cause of the defect was not determined. Subsequent research by Lemaire and Wollmann, and by Majeran et al., demonstrated that the ac46 locus affects expression of the chloroplast atpH and atpI genes encoding subunits of chloroplast ATP synthase.

Levine RP, Volkmann D (1961) Mutants with impaired photosynthesis in Chlamydomonas reinhardi. Biochem Biophys Res Commun 6:264-269

Levine RP, Goodenough UW (1970) The genetics of photosynthesis and of the chloroplast in Chlamydomonas reinhardi. Annu Rev Genet 4:397-408

Smyth RD, Martinek GW, Ebersold WT (1975) Linkage of six genes in Chlamydomonas reinhardtii and the construction of linkage test strains. J Bacteriol 124:1615-1617

Lemaire C, Wollman FA (1989) The chloroplast ATP synthase in Chlamydomonas reinhardtii. II. Biochemical studies on its biogenesis using mutants defective in photophosphorylation. J Biol Chem 264:10235-10242

Dutcher SK, Power J, Galloway RE, Porter ME (1991) Reappraisal of the genetic map of Chlamydomonas reinhardtii. J Hered 82:295-301

Majeran W, Olive J, Drapier D, Vallon O, Wollman FA (2001) The light sensitivity of ATP synthase mutants of Chlamydomonas reinhardtii. Plant Physiol 126:421-433

Locus:
AC46
Chromosome:
15

CC-705 ac46 mt+

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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