CC-735 y1 mt-
$30.00
From Itzhak Ohad, Hebrew University of Jerusalem, July 1979
Phenotype: yellow in the dark
This is Sager’s original y1 strain, as used by Ohad and others in their extensive series of experiments on Chlamydomonas greening. As y1 strains go, it seems to be reasonably stable, but users are advised to subclone for a good yellow-in-the-dark phenotype before using, since we maintain the strain in continuous light without selection for the yellow character. This strain can grow on nitrate.
CC-3992 is an equivalent strain, obtained by Hoober from Ohad. CC-1168 is a more stable allele at the same locus, and CC-1179 and CC-1180 contain a stable but temperature-conditional allele.
Sager R, Palade GE (1954) Chloroplast structure in green and yellow strains of Chlamydomonas. Exp Cell Res 7:584-588
Ohad I, Siekevitz P, Palade GE (1967a) Biogenesis of chloroplast membranes. II. Plastid differentiation during greening of a dark-grown algal mutant (Chlamydomonas reinhardi). J Cell Biol 35:553-584
Ohad I, Siekevitz P, Palade GE (1967b) Biogenesis of chloroplast membranes. II. Plastid differentiation during greening of a dark-grown algal mutant (Chlamydomonas reinhardi). J Cell Biol 35:553-584
Ford C, Wang W (1982) Instability at the y-1 locus of Chlamydomonas reinhardtii. Mol Gen Genet 187:286-290
Bednarik DP, Hoober JK ( 1985) Synthesis of Chlorophyllide b from Protochlorophyllide in Chlamydomonas reinhardtii y-1. Science 230:450-453
Dutcher SK, Power J, Galloway RE, Porter ME (1991) Reappraisal of the genetic map of Chlamydomonas reinhardtii. J Hered 82:295-301
White RA, Hoober JK (1994) Biogenesis of Thylakoid Membranes in Chlamydomonas reinhardtii y1 (A Kinetic Study of Initial Greening). Plant Physiol 106:583-590