CC-742 FUD2 (petB) mt+
$30.00
From Pierre Bennoun, Institut de Biologie Physico-Chimique, September 1979
Phenotype: requires acetate
This is a chloroplast mutant deficient in the cytochrome b6/f complex. It was obtained by Bennoun after treatment with 5-fluorodeoxyuridine.
Finazzi et al. showed that the lesion in FUD2 is a 36 bp duplication in petB, producing a 12 amino acid duplication in the cd loop of cytochrome b6. The mutant protein can assemble into the cytochrome b6/f complex, but these complexes show slower electron transfer and increased degradation in aging cells.
Finazzi G, Büschlen S, de Vitry C, Rappaport F, Joliot P, Wollman FA (1997) Function-directed mutagenesis of the cytochrome b6f complex in Chlamydomonas reinhardtii: involvement of the cd loop of cytochrome b6 in quinol binding to the Q(o) site. Biochemistry 36:2867-2874
Finazzi G, Büschlen S, de Vitry C, Rappaport F, Joliot P, Wollman FA (1997) Function-directed mutagenesis of the cytochrome b6f complex in Chlamydomonas reinhardtii: involvement of the cd loop of cytochrome b6 in quinol binding to the Q(o) site. Biochemistry 36:2867-2874