CC-744 ac-u-beta (psbA deletion) mt+
$30.00
Doug Rabert in Boynton-Gillham laboratory, Duke University, November 1979
Phenotype: requires acetate
This mutant was isolated in an arg2/arg7 diploid strain by Doug Rabert in 1979. Cells were pregrown in 0.5 mM 5-fluorodeoxyuridine in liquid cultures, concentrated, and irradiated with 2 minutes X-ray @ 6 krad/min. The irradiated cells were plated on 2 mM arsenate and arsenate-tolerant (non-photosynthesizing) clones were selected.
This strain is a mt+ derivative, obtained by successive crosses to CC-125 wild type of a mutant isolated in an arg2/arg7 diploid strain. This strain is not arginine-requiring. The mutant shows symmetrical 9 kb deletions in both copies of the psbA gene.
This strain can be used as a recipient for transformation with the psbA gene.
Myers AM, Grant DM, Rabert DK, Harris EH, Boynton JE, Gillham NW (1982) Mutants of Chlamydomonas reinhardtii with physical alterations in their chloroplast DNA. Plasmid 7:133-151