CC-803 lf2 mt+
$30.00
From George Witman, Princeton University, December 1979
This mutant has abnormally long flagella, and swims slowly. The flagella are slow to regenerate after deflagellation.
This is the original lf2 mutant, isolated by McVittie by nitrosoguanidine mutagenesis in the 137c wild type background, and was originally designated NG13. Other alleles are known at this locus: see CC-2287, CC-2288, and CC-2973.
The LF2 locus corresponds to the CDKI1 gene encoding a novel cyclin-dependent kinase required for control of flagellar length. The protein is found predominantly, if not exclusively, in the cell body in punctate structures call the Length Regulatory Complex (LRC).
Barsel SE, Wexler DE, Lefebvre PA (1988) Genetic analysis of long-flagella mutants of Chlamydomonas reinhardtii. Genetics 118:637-648
Tam LW, Wilson NF, Lefebvre PA (2007) A CDK-related kinase regulates the length and assembly of flagella in Chlamydomonas. J Cell Biol 176:819-829