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CC-5113 nphp4 mt+ [79D8]

Name: CC-5113 nphp4 mt+ [79D8]
SKU: CC-5113
Availability: InStock

$30.00

From Junya Awata, Witman lab, University of Massachusetts Medical School, February 2015

The nphp4/drc3 strain, B1179 (nphp4-1, drc3-1, arg7, ARG7, mt+) was generated by insertional mutagenesis of H11 (arg7, mt+) with the pARG7.8 plasmid and crossed sequentially to the wild-type strains CC-124 and 137c mt+ (Witman lab strain, probably of same origin as CC-125). 79D8 was created by transformation of the crossed B1179 with DRC3.

Mutant cell lacks entire NPHP4 and has defects in swimming path and flagellar regeneration rate.

Awata J, Takada S, Standley C, Lechtreck KF, Bellvé KD, Pazour GJ, Fogarty KE, Witman GB (2014) NPHP4 controls ciliary trafficking of membrane proteins and large soluble proteins at the transition zone. J Cell Sci. 127:4714-27

CC-5113 nphp4 mt+ [79D8]

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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