* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-5115 NPHP4-HAC mt- [17HA81]
$30.00
From Junya Awata, Witman lab, University of Massachusetts Medical School, February 2015
Transformation of nphp4/drc3 strain, B1179 (nphp4-1, drc3-1, arg7, ARG7, mt+) with NPHP4-HAC (gene encoding nephrocystin-4 tagged with 3xHA peptides on its C-terminus) followed by two crosses to wild-type strains CC-124 and 137c mt+ (Witman lab strain, probably of same origin as CC-125) and transformation of selected offspring with DRC3.
Awata J, Takada S, Standley C, Lechtreck KF, Bellvé KD, Pazour GJ, Fogarty KE, Witman GB (2014) NPHP4 controls ciliary trafficking of membrane proteins and large soluble proteins at the transition zone. J Cell Sci. 127:4714-27