* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
P-135 cpDNA EcoRI-KpnI (3.0 kb) atpB mutant
$30.00
Boynton-Gillham laboratory, Duke University, 1986
Chloroplast DNA EcoRI-KpnI (3.0 kb) in pUC18; atpB from CC-440, containing the ac-u-c-2-29 mutation (K154N)
host strain: JM103
amp resistant
More information
Strain: C. reinhardtii CC-440 ac-u-c-2-29
Insert: KpnI-EcoRI (3.0 kb)
Genes present: atpB
Vector: pUC18
Bacterial host strain: JM103
Selectable marker: amp-r, white on X-gal
Origin: Jeff Woessner in Boynton/Gillham lab, 1990
Robertson D, Woessner JP, Gillham NW, Boynton JE (1989) Molecular characterization of two point mutants in the chloroplast atpB gene of the green alga Chlamydomonas reinhardtii defective in assembly of the ATP synthase complex. J Biol Chem 264:2331-2337