* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
P-157 cpDNA BamHI-BglI (10 kb) psbA DCMU4
$30.00
From Jean-David Rochaix to Boynton-Gillham laboratory, 1987
Chloroplast DNA BamHI-BglII 10 kb fragment in pUC18; subclone of Bam 4; psbA from DCMU4 mutant (= CC-2473).
host strain: 521
amp resistant
More information
Strain: DCMU4, equivalent to CC-2473
Insert: BamHI-BglI (10 kb)
Note: Bam-Bgl digests consistently yield a small amount of vector + insert, with most of the DNA uncut
Genes present: psbA
Vector: pUC18
Bacterial host strain: 521
Selectable marker: amp-r, white on X-gal
Origin: Jean-David Rochaix to Boynton/Gillham lab, 1987
See Erickson et al., PNAS 81, 3617 (1984)
C. reinhardtii DCMU4 contains a ser->ala alteration in the 5th exon of psbA at position 264 conferring resistance to DCMU and atrazine.