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P-161 cpDNA EcoRI 36/37 (0.9 kb) nr-u-2-1

Name: P-161 cpDNA EcoRI 36/37 (0.9 kb) nr-u-2-1
SKU: P-161
Availability: InStock

$30.00

Boynton-Gillham laboratory, Duke University, 1987

Chloroplast DNA EcoRI 36/37 (0.9 kb) in pUC8; contains 16S rRNA gene (3′ end) from CC-86 neamine/kanamycin resistant mutant nr-u-2-1

host strain: JM83
amp resistant

More information

Strain: CC-86 nr-u-2-1 mt+

Insert: EcoRI 36/37 (0.9 kb)

Genes present: 16S rRNA (3′ end)

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1987

Contains A->G at bp 1340 in C. reinhardtii 16S rRNA gene which produces high level resistance to neamine and kanamycin.
Harris et al., Genetics 123, 281 (1989).

Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292

P-161 cpDNA EcoRI 36/37 (0.9 kb) nr-u-2-1

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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