* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
P-173 cpDNA EcoRI 14 (5.8 kb) rbcL ts mutant
$30.00
From Robert Spreitzer to Boynton-Gillham laboratory, 1988
Chloroplast DNA EcoRI 14 (5.8 kb) in pBR329; rbcL containing Spreitzer 68-4PP ts mutation (L290F).
host strain: JM83
amp resistant
More information
Strain: CC-2338 68-4PP, rbcL mutant
Insert: EcoRI 14 (5.8 kb)
Genes present: psaB, rbcL, atpA (part)
Vector: pBR329
Bacterial host strain: JM83
Selectable marker: amp-r, tet-r
Origin: Bob Spreitzer, 1988 to Boynton/Gillham lab
The 68-4PP mutant has a ts photosynthesis deficient phenotype due to a phe for leu substitution at residue 290 in the Rubisco large subunit.
References:
Chen et al. (1993) Plant Physiol 101, 1189-1194
Chen et al. (1988) Proc. Natl. Acad. Sci. USA 85, 4696-4699