P-179 cpDNA BamHI-EcoRI (4.1 kb) er-u-37

$30.00

Boynton-Gillham laboratory, Duke University, 1988

Chloroplast DNA BamHI-EcoRI (4.1 kb) in pUC18; 5′ end of 23S rRNA gene from CC-67 erythromycin resistant mutant er-u-37

host strain: TG-1
amp resistant

 

More information

Strain: CC-67 er-u-37

Insert: BamHI-EcoRI (4.1 kb)
overlap of Eco 3/4 and Bam 11/12
Genes present: 23S rRNA (5′ end)

Vector: pUC18

Bacterial host strain: TG-1

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1988

P-179

The er-u-37 mutation is a G->A change at bp 2066 in C. reinhardtii 23S rRNA
(corresponding to E. coli 2057) which produces resistance to erythromycin.
See Harris et al., Genetics 123, 281 (1989).

Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292