P-183 cpDNA BamHI 11/12 (7.0 kb) sr spr er

$30.00

Boynton-Gillham laboratory, Duke University, 1988

Chloroplast DNA BamHI 11/12 (7.0 kb) in pUC18; 16S through 5′ end of 23S rRNA gene; contains sr-u-2-60, spr-u-1-6-2, and er-u-37 mutations

host strain: JM83
amp resistant

 

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Strain: CC-227 spr-u-1-6-2 sr-u-2-60 er-u-37

Insert: BamHI 11/12 (7.0 kb)

Genes present: 16S rRNA, 23S rRNA (5’end)

Vector: pUC18

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1988

P-183

See Harris et al., Genetics 123, 281 (1989) for mutations, Newman et al., Genetics 126, 875 (1990) for use of this plasmid in transformation experiments.
Note: by selecting cells transformed with this plasmid simultaneously for spectinomycin and streptomycin or erythromycin resistance one essentially eliminates the possibility of recovering spontaneous antibiotic resistant mutants.
However, cells carrying both spr-u-1-6-2 and sr-u-2-60 have been found to be deficient in chloroplast protein synthesis and have impaired photosynthetic performance under high iradiance conditions, consistent with reduced levels of D1 synthesis.
Thus if selection for antibiotic resistance is to be used specifically for cotransformation, we recommend using P-228 (spr-u-1-6-2 alone) instead.