P-192 cpDNA BamHI-HindIII (1.6 kb) er-u-1a

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Boynton-Gillham laboratory, Duke University, 1989

Chloroplast DNA BamHI-HindIII (1.6 kb) in pUC119; 3′ end of 23S rRNA gene from CC-64, erythromycin resistant mutant er-u-1a

host strain: probably TG-1
amp resistant

 

More information

Strain: CC-64 er-u-1a

Insert: BamHI-HindIII (1.6 kb)

Genes present: 23S rRNA (3’end)

Vector: pUC119

Bacterial host strain: probably TG-1

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1988

P-192

The mutation in er-u-1a is a C->T change at bp 2622 in the C. reinhardtii 23S rRNA gene (corresponding to E. coli 2611), which produces erythromycin resistance.
See Harris et al., Genetics 123, 281 (1989).

Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292