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P-202 cpDNA BamHI 6

Name: P-202 cpDNA BamHI 6
SKU: P-202
Availability: InStock

$30.00

Boynton-Gillham laboratory, Duke University, 1989

Chloroplast DNA BamHI 6 (13.5 kb) in pBR322; contains 3′ end of rps2 rpoA, psbB, psbN, and psbH; P-21 is the same insert in pBR313

host strain: DH1?
amp resistant

More information

Strain: CC-126 (a strain no longer in the stock collection)
CC-126 was derived from a cross of an ac20 cr1 double mutant to a wild-type strain.
It was a tetrad product with the wild-type alleles of both these mutations.

Insert: BamHI 6 (ca. 13.4 kb)

Genes present: rps2-C terminus (part), rpoA, pbB, psbT, psbN, psbH

Vector: pBR322

Bacterial host strain: DH1?

Selectable marker: amp-r

Origin: Jeff Woessner in Boynton/Gillham lab, 1990

This map is from the original file for the plasmid and was drawn before the complete sequence was known.
As indicated above in the “Genes present” line, several other genes were subsequently identified in this fragment.

P-202 cpDNA BamHI 6

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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