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P-206 cpDNA PstI-XhoI (8.75 kb) atpE

Name: P-206 cpDNA PstI-XhoI (8.75 kb) atpE
SKU: P-206
Availability: InStock

$30.00

Boynton-Gillham laboratory, Duke University, 1989

Chloroplast DNA PstI-XhoI (8.75 kb) in pUC18, from CC-125 wild type; contains atpE and 3′ end of rps7

host strain: JM83
amp resistant

More information

Strain: CC-125 wild type mt+

Insert: PstI-(XhoI) SalI (ca. 9 kb); conflict in original data regarding size
This is the overlap between Pst 8 and Xho 5, cloned into the Pst-Sal sites of the plasmid.
The Xho site was lost in the cloning process.
Genes present: atpE, rps7 (3’end), psbA exons 1 and 2

Vector: pUC18

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Niki Robertson in Boynton/Gillham lab, 1989

This map is from the original file for the plasmid and was drawn before the complete sequence was known.
Also, the distance on the map is larger than the stated size of the insert.

P-206 cpDNA PstI-XhoI (8.75 kb) atpE

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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