P-210 cpDNA atpB, aadA BamHI-EcoRI (7.3 kb)

$30.00

Boynton-Gillham laboratory, Duke University, 1989

Chloroplast DNA BamHI-EcoRI (7.3 kb) in pUC8; contains aadA inserted downstream of atpB

host strain: JM83
amp resistant

 

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Strain: cw15

Insert: BamHI-EcoRI (7.3 kb)

Genes present: aadA, C. reinhardtii atpB

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton-Gillham lab, 1989

P-210

This plasmid was derived from one constructed in the laboratory of Lawrence Bogorad, in which the NPTII gene under control of the maize rbcL promoter was inserted downstream of atpB (Blowers et al. 1989, Plant Cell 1, 123-132). The NPTII-rbcL fragment was replaced with the E. coli aadA gene to create P-210.