P-214 cpDNA HincII-KpnI (1.47 kb) rps12

$30.00

Boynton-Gillham laboratory, Duke University, 1989

Chloroplast DNA HincII-KpnI (1.47 kb) in pUC119, from CC-125 wild type, subclone of EcoRI 17; contains wild-type rps12; P-175 is the same insert in pUC118

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+

Insert: HincII-KpnI (1.47 kb)

Genes present: rps12

Vector: pUC119

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Paul Liu in Boynton/Gillham lab, 1989

P-175

Insert contains the 402 bp rps12 coding sequence plus 590 bp upstream and 480 bp downstream.
Reference: Liu et al., J. Biol. Chem. 264, 16100-16108 (1989).
P-214 contains the same insert cloned into pUC119

Liu XQ, Gillham NW, Boynton JE (1989) Chloroplast ribosomal protein gene rps12 of Chlamydomonas reinhardtii. Wild-type sequence, mutation to streptomycin resistance and dependence, and function in Escherichia coli. J Biol Chem 264:16100-16108