P-216 cpDNA HincII-KpnI (1.47 kb) rps12 mutant

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Boynton-Gillham laboratory, Duke University, 1989

Chloroplast DNA HincII-KpnI (1.47 kb) in pUC18, from CC-125 wild type, subclone of EcoRI 17; contains rps12 in which Arg85 has been mutated to stop codon

host strain: JM83
amp resistant

 

More information

Strain: CC-125 wild type mt+ (from plasmid P-70)

Insert: HincII-KpnI (1.47 kb)

Genes present: rps12

Vector: pUC18

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Paul Liu in Boynton/Gillham laboratory, 1989

P-216

Insert = rps12 mutagenized in vitro (CGA arg at aa 85 changed to TGA stop codon) and cloned into SmaI site of pUC18. See Liu et al., J. Biol. Chem. 264, 16100 (1989).

Liu XQ, Gillham NW, Boynton JE (1989) Chloroplast ribosomal protein gene rps12 of Chlamydomonas reinhardtii. Wild-type sequence, mutation to streptomycin resistance and dependence, and function in Escherichia coli. J Biol Chem 264:16100-16108