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P-230 cpDNA AatII (0.28 kb) SDR probe

Name: P-230 cpDNA AatII (0.28 kb) SDR probe
SKU: P-230
Availability: InStock

$30.00

Boynton-Gillham laboratory, Duke University, 1989

Chloroplast DNA 0.28 kb AatII fragment in pUC18, from BamHI 11/12, CC-125 wild type; spacer region between 16S and 23S rRNA genes; used as a probe for short dispersed repeat sequences in the chloroplast genome

host strain: JM83
amp resistant

More information

Strain: CC-125 wild type mt+

Insert: AatII (0.28 kb)

Vector: pUC18

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Scott Newman in Boynton/Gillham lab, 1990-

The insert is subclone of Bam 11/12, located within the spacer region between the 16S and 23S rRNA genes and containing short dispersed repeat elements. The AatII fragment was treated with Klenow to generate blunt ends and cloned into the SmaI site of pUC18. The AatII and SmaI sites have been lost but the insert can be excised using restriction sites in the pUC18 polylinker.

P-230 cpDNA AatII (0.28 kb) SDR probe

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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