P-261 cpDNA EcoRI (5.51 kb) er-u-1a

$30.00

Boynton-Gillham laboratory, Duke University, 1990

Chloroplast DNA EcoRI (5.51 kb) in pUC8 contains 3′ end of psbA, 5S, and 3′ exon of 23S with er-u-1a mutation from CC-64

host strain: JM109
amp resistant

 

More information

Strain: CC-64 er-u-1a mt+

Insert: EcoRI (5.51 kb)

Genes present: psbA 3′ end, 5S rRNA, 23S rRNA (small exon)

Vector: pUC8

Bacterial host strain: JM109

Selectable marker: amp-r, white on X-gal

Origin: Amnon Lers in Boynton/Gillham lab, 1990

P-261

The er-u-1a mutation is a C->T change at bp 2622 in the C. reinhardtii 23S rRNA gene (Lemieux numbering, equivalent to E. coli 2611), which confers erythromycin resistance in Chlamydomonas.

This is a subclone of P-259B. The insert contains sequences from the EcoRI site internal to psbA up to the EcoRI site in KS+, thus including some sequences of the SK+ polylinker beyond the original XhoI border of the insert in P-259B. Orientation of psbA is from the pUC8 Eco site toward the pUC8 HindIII site.