P-262 cpDNA EcoRI (4.0 kb) er-u-1a

$30.00

Boynton-Gillham laboratory, Duke University, 1990

Chloroplast DNA EcoRI (4.0 kb) in pUC8; contains 3′ end of psbA, 5S, and 3′ exon of 23S with er-u-1a mutation from CC-64; 1.5 kb deletion in spacer between psbA and 5S

host strain: JM109
amp resistant

 

More information

Strain: CC-64 er-u-1a mt+

Insert: EcoRI (4.0 kb)

Genes present: psbA (exon 5), 5S rRNA, 23S rRNA (small exon)

Vector: pUC8

Bacterial host strain: JM109

Selectable marker: amp-r, white on X-gal

Origin: Amnon Lers in Boynton/Gillham lab, 1990

P-262

This is a subclone of P-261. The three KpnI fragments including the BamHI site (approx. 1.5 kb) located between the psbA and 5S genes were deleted, and the remaining two Kpn ends were religated, leaving only one KpnI site. The insert appears slightly smaller than 4 kb.

The er-u-1a mutation is a C->T change at bp 2622 in C. reinhardtii 23S rRNA gene (Lemieux numbering, equivalent to E. coli 2611), which confers erythromycin resistance in Chlamydomonas.