* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
P-263 cpDNA BamHI-HindIII (4.8 kb) nr spr
$30.00
Boynton-Gillham laboratory, Duke University, 1990
Chloroplast DNA BamHI-HindIII (4.8 kb) in pUC18; 16S rRNA gene from CC-245, containing the spr-u-1-H-4 and nr-u-2-1 mutations
host strain: JM83
amp resistant
More information
Strain: CC-245 nr-u-2-1 spr-u-1-H-4
Insert: BamHI-HindIII (4.8 kb)
Genes present: 16S rRNA, 23S rRNA (5’ end)
Vector: pUC18
Bacterial host strain: JM83
Selectable marker: amp-r, white on X-gal
Origin: Scott Newman in Boynton/Gillham lab, 1990
The nr-u-2-1 mutation is an A->G change at bp 1340 in the 16S rRNA gene, and the spr-u-1-H-4 mutations is an A->G change at bp 1123 in the same gene, conferring resistance to neamine/kanamycin and spectinomycin, respectively. See Harris et al., Genetics 123: 281-292 (1989).