* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
P-268 cpDNA KpnI (2.4 kb) spr-u-1-6-2
$30.00
Boynton-Gillham laboratory, Duke University, 1990
Chloroplast DNA KpnI (2.4 kb) in pUC18; 16S rRNA gene from CC-110, spectinomycin resistant mutant spr-u-1-6-2
host strain: JM83
amp resistant
More information
Strain: CC-110 spr-u-1-6-2 mt+
Insert: KpnI (2.4 kb)
Genes present: 16S rRNA
Vector: pUC18
Bacterial host strain: JM83
Selectable marker: amp-r, white on X-gal
Origin: Scott Newman in Boynton/Gillham lab, 1990
The spr-u-1-6-2 mutation is an A->G change at bp 1123 in the C. reinhardtii 16S rRNA gene which causes the loss of an AatII restriction site and confers high level spectinomycin resistance. See Harris et al., Genetics 123: 281-292 (1989).