* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
P-269 cpDNA ClaI (3.2 kb) psbA
$30.00
Boynton-Gillham laboratory, Duke University, 1990
Chloroplast DNA ClaI (3.2 kb) in BSKS+; psbA 5′ flank, exons 1 and 2.
host strain: JM109
amp resistant
More information
Strain: CC-64 er-u-1a mt+
Insert: ClaI (3.2 kb)
Genes present: psbA 5’ flank, exons 1 and 2
Vector: BSKS+
Bacterial host strain: JM109
Selectable marker: amp-r, white on X-gal
Origin: Amnon Lers in Boynton/Gillham lab, 1990
The insert is a subclone of Xho8 cloned in BSKS+ (P-259) and contains sequences from ClaI to XhoI in the KS polylinker followed by psbA sequences from XhoI up to a ClaI site near exon 2 cloned into the ClaI site of BSKS+. Orientation of psbA is from KS Kpn site toward KS SacI site. Insert sequences in this fragment are identical to wild-typ