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P-291 cpDNA 23S rRNA 5′ exon
$30.00
From Scott Newman, Boynton-Gillham laboratory, October 1990
chloroplast DNA XhoI-SmaI (2.8 kb) in BSKS+, 5′ exon of the 23S rRNA gene
host strain: JM109
amp resistant
More information
Strain: partly derived from CC-125 and partly from the Rochaix cw15 strain
Insert: XhoI-SmaI (2.6 kb)
Genes present: 23S rRNA, 5′ exon
Vector: BSKS+
Bacterial host strain: JM109
Selectable marker: amp-r, white on X-gal
Origin: Scott Newman in Boynton/Gillham lab, 1990
The insert was constructed by ligating the 1.1 kb XhoI-BamHI fragment from Bam 14/15 (P-14) to the 1.5 kb BamHI-SmaI fragment from Bam 11/12 (P-59), resulting in a 2.6 kb Xho-Sma fragment.