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P-297 cpDNA Kpn I (3.1 kb) psbA R238K DCMU4
$30.00
Boynton-Gillham laboratory, Duke University, 1990
Chloroplast DNA Kpn I (3.1 kb) in BSKS+; psbA exons 4 and 5 with R238K mutation in exon 4 and DCMU4 (S264K) in exon 5, from CC-2473
host strain: JM109
amp resistant
More information
Strain: CC-2473 DCMU4
Insert: KpnI (3.1 kb)
Genes present: psbA exons 4 and 5
Vector: BSKS+
Bacterial host strain: JM109
Selectable marker: amp-r, white on X-gal
Origin: Amnon Lers in Boynton/Gillham lab, 1990
This plasmid was derived from P-260 by in vitro mutagenesis of the arg residue at aa 238 to lys.
DCMU4 is a ser->ala mutation at aa 264 in the C. reinhardtii psbA 5th exon, conferring resistance to DCMU and atrazine.