P-300 cpDNA Kpn I (3.1 kb) psbA R238K

$30.00

Boynton-Gillham laboratory, Duke University, 1990

Chloroplast DNA Kpn I (3.1 kb) in BSKS+; psbA exons 4 and 5 with R238K mutation in exon 4

host strain: JM109
amp resistant

 

More information

Insert: KpnI (3.1 kb)

Genes present: psbA (exons 4 and 5)

Vector: BSKS+

Bacterial host strain: JM109

Selectable marker: amp-r, white on X-gal

Origin: Amnon Lers in Boynton/Gillham lab, 1990

P-300

A 0.9 kb Eco RI fragment from P-260A (Eco sites located within 4th intron of psbA and BSKS polylinker) containing psbA exon 4 was mutated in vitro at position 238 arg -> lys and cloned in the EcoRI site of P-299 (2.3 kb KpnI-EcoRI fragment containing wild type sequences of psbA intron 4 and exon 5 cloned into BSKS+), yielding a 3.1 kb Kpn fragment inserted into BSKS+. This plasmid has the psbA Kpn 3.1 kb fragment with only the arg -> lys mutation.